When you register multiple listeners for the same event using the on method, Node.js stores them in the order they were added. When the event is emitted, all listeners are called synchronously, one after another, in this order. This means the first listener registered runs first, followed by the next, and so on. If a listener is removed using removeListener or off, it will no longer respond to future emissions of that event. This mechanism gives you fine control over how your application responds to events and allows you to adjust event flow dynamically at runtime.